Composite

Part:BBa_K3812009

Designed by: Guanghui Dong   Group: iGEM21_CHINA-FAFU   (2021-10-01)


Code UDP-Glucose-Pyrophosphorylase

Paox1 Promoter can regulate the initiation of UGP transcription, and CYC1 can terminate UGP transcription. This compatible part can express UGP in the chassis organism-Pichia pastoris.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1282
    Illegal EcoRI site found at 2405
    Illegal EcoRI site found at 2825
    Illegal PstI site found at 1898
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1282
    Illegal EcoRI site found at 2405
    Illegal EcoRI site found at 2825
    Illegal NheI site found at 1559
    Illegal PstI site found at 1898
    Illegal NotI site found at 3230
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1282
    Illegal EcoRI site found at 2405
    Illegal EcoRI site found at 2825
    Illegal BglII site found at 1
    Illegal BamHI site found at 3838
    Illegal BamHI site found at 5102
    Illegal XhoI site found at 1403
    Illegal XhoI site found at 2245
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1282
    Illegal EcoRI site found at 2405
    Illegal EcoRI site found at 2825
    Illegal PstI site found at 1898
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1282
    Illegal EcoRI site found at 2405
    Illegal EcoRI site found at 2825
    Illegal PstI site found at 1898
    Illegal NgoMIV site found at 1408
    Illegal AgeI site found at 2273
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None